Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

Disposal of Mine Wastes Containing Pb and Zn Near the Ocean: An Assessment of Associated Environmental Implications in the Arctic

The past two decades have witnessed an increase in the exploration for, and development of, mineral resources at Arctic latitudes. Such effort has resulted in the commencement of production at a number of different localities in close proximity to the ocean. Since proximity to a source of transportation for the conveyance of concentrates to the market is a primary requirement governing the economic decisions for the mine to proceed, it is logical that ore deposits near a coastline are primary targets for development.

Closely tied to coastal mining developments is the need to dispose of tailings and waste rock in the most economical manner. Prior to the advent of heightened public awareness and environmental concern, and the enactment of pollution legislation, disposal practices were largely based upon convenience. Currently, however, proposed disposal and operating practices receive closer scrutiny, with a view to providing adequate protection for aquatic resources and habitat. This paper summarizes some of the features of three Arctic mines, wastes from which enter the marine environment, and compares the disposal practices used at each to measures of environmental change as indicated by metal concentrations in various media.     

Environmental auditor qualifications: Meeting new,rigorous demands for quality audits

“Ah, Mr. Cahill, I wonder if you could come back to the auditor's team room. It seems one of your auditors has fallen asleep for the second time this week. I know you had a grueling trip getting here, but this is embarrassing to everyone”. So went an audit I led some time ago. It was not a fun experience, and it has left some scars. There has been much discussion lately in the profession about certifying environmental (health and safety) auditors against certain performance criteria. But how does one evaluate for such important measures as physical stamina?. This article discusses the qualifications that environmental auditors should have if they are to lead or conduct a quality audit. It becomes evident after reviewing these expectations that certifying auditors through a traditional written examination might ignore some of the most important skills an auditor should possess, especially in working with managers throughout an organization. Before certifying organizations progress too far down the restrictive written-exam road, they should determine how these other skills are to be assessed. No amount of book learning can overcome the drawbacks of a poor or combative interviewer or even an exhausted auditor.     

The Role of Foreign Direct Investment in Urban Environmental Management: Some Evidence from Hanoi, Vietnam

In recent years flows of foreign direct investment (FDI) to developing countries have rapidly been increasing. It is now an important contributor to the national economy and urban growth in scores of developing countries. Rapid urbanization in these countries is leading to many problems in the cities. Existing urban infrastructure and municipal services have been unable to cope with the increased demand arising from growing population and rapid economic growth. Consequently, the environment in these cities has deteriorated alarmingly. This paper assesses the role of FDI in urban environmental management (UEM) by analyzing the linkages between them for Hanoi City in Vietnam. The paper's analysis and findings are presented under three main themes: one, FDI, employment and income; two, FDI and environmental degradation; and three, potential of utilizing FDI for the provision of urban environmental infrastructure and services (UEI&S) in order to improve the urban environment. Both primary and secondary source data have been used. Policy implications of the research findings are drawn from the perspective of sustainable development.     

The Use of Natural Taraxacum officinale Wigg. Populations for Assessing the State of Technogenically Disturbed Areas

Russian Journal of Ecology -     

Soil nitrate nitrogen dynamics after biosolids application in a tobosagrass desert grassland

Dormant-season application of biosolids increases desert grass production more than growing season application in the first growing season after application. Differential patterns of NO3-N (plant available N) release following seasonal biosolids application may explain this response. Experiments were conducted to determine soil nitrate nitrogen dynamics following application of biosolids during two seasons in a tobosagrass [Hilaria mutica (Buckl.) Benth.] Chihuahuan Desert grassland. Biosolids were applied either in the dormant (early April) or growing (early July) season at 0, 18, or 34 dry Mg ha(-1). A polyester-nylon mulch was also applied to serve as a control that approximated the same physical effects on the soil surface as the biosolids but without any chemical effects. Supplemental irrigation was applied to half of the plots. Soil NO3-N was measured at two depths (0-5 and 5-15 cm) underneath biosolids (or mulch) and in interspace positions relative to surface location of biosolids (or mulch). Dormant-season biosolids application significantly increased soil NO3-N during the first growing season, and also increased soil NO3-N throughout the first growing season compared to growing-season biosolids application in a year of higher-than-average spring precipitation. In a year of lower-than-average spring precipitation, season of application did not affect soil NO3-N. Soil NO3-N was higher at both biosolids rates for both seasons of application than in the control treatment. Biosolids increased soil NO3-N compared to the inert mulch. Irrigation did not significantly affect soil NO3-N. Soil NO3-N was not significantly different underneath biosolids and in interspace positions. Surface soil NO3-N was higher during the first year of biosolids application, and subsurface soil NO3-N increased during the second year. Results showed that biosolids rate and season of application affected soil NO3-N measured during the growing season. Under dry spring-normal summer precipitation conditions, season of application did not affect soil NO3-N; in contrast, dormant season application increased soil NO3-N more than growing season application under wet spring-dry summer conditions.     

Prenatal diagnosis and molecular cytogenetic characterisation of a small de novo interstitial duplication 16q11.2-q13

We describe the first prenatally detected case of a small de novo interstitial duplication of chromosome 16q. This chromosomal aberration is extremely rare. Amniocentesis was indicated by advanced maternal age only. Ultrasound examinations of the foetus showed no abnormalities. Conventional and molecular cytogenetic analyses on cultured amniocytes by comparative genomic hybridisation (CGH) and fluorescence in situ hybridisation (FISH) using partial chromosome paints and a locus-specific YAC clone revealed a de novo direct duplication of the chromosomal region 16q11.2-q13 leading to a partial trisomy 16q (46,XX,dup(16)(q11.2q13)). There are only five postnatal reports of comparable duplications involving this chromosomal region. These patients presented with little or no associated dysmorphic features but with significant neurodevelopmental delay and severe behavioural problems. After genetic counselling, the parents opted for termination of pregnancy. Post-mortem examination showed slight facial dysmorphic signs, minor dysgenesis of the ovaries and an atypical outflow of the arteria thyroidea ima. Copyright © 2006 John Wiley & Sons, Ltd.     

Decision support systems for stored water quality: 2. An example application

The components of an environmental decision support system (EDSS) were outlined in Part 1 of this paper. Here, an example application is given using a range of data from the database in order to assess the utility of one specific model chosen from the modelbase: the one-dimensional thermal stratification model, EDD1. The model is applied to a range of lake types worldwide.     

Exposure to radiation from global system for mobile communications at 1,800 MHz significantly changes gene expression in rat hippocampus and cortex

We have earlier shown that radio frequency electromagnetic fields can cause significant leakage of albumin through the blood–brain barrier of exposed rats as compared to non-exposed rats, and also significant neuronal damage in rat brains several weeks after a 2 h exposure to a mobile phone, at 915 MHz with a global system for mobile communications (GSM) frequency modulation, at whole-body specific absorption rate values (SAR) of 200, 20, 2, and 0.2 mW/kg. We have now studied whether 6 h of exposure to the radiation from a GSM mobile test phone at 1,800 MHz (at a whole-body SAR-value of 13 mW/kg, corresponding to a brain SAR-value of 30 mW/kg) has an effect upon the gene expression pattern in rat brain cortex and hippocampus—areas where we have observed albumin leakage from capillaries into neurons and neuronal damage. Microarray analysis of 31,099 rat genes, including splicing variants, was performed in cortex and hippocampus of 8 Fischer 344 rats, 4 animals exposed to global system for mobile communications electromagnetic fields for 6 h in an anechoic chamber, one rat at a time, and 4 controls kept as long in the same anechoic chamber without exposure, also in this case one rat at a time. Gene ontology analysis (using the gene ontology categories biological processes, molecular functions, and cell components) of the differentially expressed genes of the exposed animals versus the control group revealed the following highly significant altered gene categories in both cortex and hippocampus: extracellular region, signal transducer activity, intrinsic to membrane, and integral to membrane. The fact that most of these categories are connected with membrane functions may have a relation to our earlier observation of albumin transport through brain capillaries.